GBA3 antibody

Catalog No. ABIN7468369

The Rabbit Polyclonal anti-GBA3 antibody has been validated for WB and IHC (p). It is suitable to detect GBA3 in samples from Human.

Quick Overview for GBA3 antibody (ABIN7468369)

Target: GBA3 (Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GBA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GBA3 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GBA3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GBA3

Target: GBA3 (Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3))

Alternative Name: glucosylceramidase beta 3 (gene/pseudogene)

Background:

Synonyms: glucosylceramidase beta 3 (gene/pseudogene) , CBG , CBGL1 , GLUC , KLRP

Background: GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM]

Molecular Weight: 54 kDa

Gene ID: 57733

UniProt: Q9H227